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Identification of antigenic sites on three hepatitis C virus proteins using phage‐displayed peptide libraries
Author(s) -
Pereboeva L. A.,
Pereboev A. V.,
Morris G. E.
Publication year - 1998
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199810)56:2<105::aid-jmv1>3.0.co;2-c
Subject(s) - ns3 , epitope , virology , antigen , biology , peptide , phage display , peptide library , virus , hepatitis c virus , antibody , microbiology and biotechnology , peptide sequence , biochemistry , gene , genetics
A novel approach to screening phage‐displayed peptide libraries has been used to identify hepatitis C virus (HCV) core, NS4 and NS5 sequences, which are antigenic in humans. Two random peptide libraries were used for screening using a mixture of HCV‐positive sera or individual antibodies to core, NS3, NS4, and NS5 HCV proteins affinity‐purified from this mixture. Sequencing of 56 selected phage clones resulted in 28 different peptide sequences and identification of seven antigenic regions, three in the core protein (19‐26, 34‐49, and 73‐83), three in the NS4 (1681‐1693, 1712‐1718, and 1726‐1736) and one in the NS5 protein (2251‐2260). No NS3‐specific peptides were identified. The immune response to core, NS4 and NS5 proteins includes a variety of linear determinants whereas epitopes on the investigated part of NS3 protein appear to be conformation‐dependant. J. Med. Virol. 56:105–111, 1998 . © 1998 Wiley‐Liss, Inc.