Premium
Human cytomegalovirus infection induces mRNA expression and secretion of plasminogen inhibitor type‐1 in endothelial cells
Author(s) -
Woodroffe Salwa B.,
Kuan Siong
Publication year - 1998
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199808)55:4<268::aid-jmv3>3.0.co;2-z
Subject(s) - human cytomegalovirus , plasminogen activator , secretion , biology , plasminogen activator inhibitor 1 , virology , messenger rna , blot , microbiology and biotechnology , northern blot , cytomegalovirus , virus , herpesviridae , gene , viral disease , endocrinology , biochemistry
The aim of the study was to investigate whether infection of endothelial cells with human cytomegalovirus (HCMV) perturbs expression and production of plasminogen activator inhibitor type‐1 (PAI‐1). mRNA expression of PAI‐1 was investigated by isolating total RNA from HCMV‐infected and control cells, followed by Northern blotting and probing with 32 P‐labelled PAI‐1 probe. Sandwich ELISA was used to investigate PAI‐1 production. HCMV induced the expression of PAI‐1‐mRNA at 2–5 days postinfection (maximum expression was at 3 days postinfection which was 40% higher than control). HCMV also induced secretion of PAI‐1 at 2–5 days postinfection. These results indicate that infection of endothelial cells with HCMV disturbs PAI‐1 expression and production in these cells. J. Med. Virol. 55:268–271, 1998 . © 1998 Wiley‐Liss, Inc.