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Consistent polymerse chain reaction–single‐strand conformation polymorphism pattern of human herpesvirus‐8 in the course of classical Kaposi's sarcoma assumes its clonal origin
Author(s) -
Juhász Attila,
Remenyik Eva,
Szarka Krisztina,
Veress György,
Hunyadi János,
Gergely Lajos
Publication year - 1998
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199804)54:4<300::aid-jmv10>3.0.co;2-j
Subject(s) - single strand conformation polymorphism , sarcoma , amplicon , polymerase chain reaction , virology , biology , kaposi's sarcoma , kaposi's sarcoma associated herpesvirus , nested polymerase chain reaction , pathogenesis , virus , microbiology and biotechnology , human herpesvirus , herpesviridae , viral disease , pathology , genetics , gene , medicine , immunology
There is emerging evidence that Kaposi's sarcoma–associated herpesvirus (KSHV or HHV‐8) has a central role in the pathogenesis of Kaposi's sarcoma (KS). The occurrence of HHV‐8 in classical KS biopsies is reported irrespective of its clinical stage (patch, plaque, nodular). HHV‐8 was detected in 25 of 28 formalin‐fixed paraffin‐embedded classical KS samples by nested polymerase chain reaction. In addition, in six patients multiple tumors were available (n = 21). Single‐strand conformation polymorphism (SSCP) analysis of the amplicons showed uniform SSCP pattern of samples belonging to the same patient regardless of whether the KS was multiplex or developed again years after the first excision. Most of the SSCP patterns were confirmed by further sequence analysis. The presence of the same sequence variant of HHV‐8 in various samples of the same patient supports the clonal origin of classical Kaposi's sarcoma. J. Med. Virol. 54:300–304, 1998 . © 1998 Wiley‐Liss, Inc.