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Human papillomavirus DNA sequences and p53 over‐expression in laryngeal squamous cell carcinomas in Northeast China
Author(s) -
Ma XiuLan,
Ueno Kazuyoshi,
Pan ZiMin,
Hi SengZhong,
Ohyama Masaru,
Eizuru Yoshito
Publication year - 1998
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199803)54:3<186::aid-jmv8>3.0.co;2-1
Subject(s) - virology , human papillomavirus , biology , dna , papillomaviridae , china , cancer research , genetics , medicine , cancer , cervical cancer , history , archaeology
One‐hundred‐two patients with laryngeal squamous cell carcinomas in Northeast China were examined for human papillomavirus (HPV) DNA by the polymerase chain reaction (PCR) coupled with Southern blot hybridization, and for p53 over‐expression by immunohistochemical staining. HPV DNAs were found in 60 cases (58.8%). HPV‐16, ‐18, ‐6, ‐11, and ‐33 DNAs were detected in 30 cases, 22 cases, 25 cases, two cases, and one case, respectively. In addition, coinfection either with HPV‐6 and ‐16 or with HPV‐6 and ‐18 was detected in 20 cases (33.3% of HPV DNA‐positive cases). p53 over‐expression was observed in 60 patients (58.8%). p53 was over‐expressed significanty in the poorly‐differentiated SCC and in patients with metastasis to lymph nodes ( P < 0.05, respectively). Both HPV DNA and p53‐expression were positive in 35 patients, and negative in 17 patients. Either HPV DNA or p53‐expression were positive in 50 patients (25 cases each). Although p53 was detected in 35 (58.3%) of HPV‐positive patients, there was no significant correlation between HPV infection and p53 over‐expression in laryngeal squamous cell carcinomas of Northeast China. J. Med. Virol. 54:186–191, 1998. © 1998 Wiley‐Liss, Inc.