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Quasispecies nature of hepatitis C virus (HCV) in patients with chronic hepatitis C with mixed HCV subtypes
Author(s) -
Toyoda Hidenori,
Fukuda Yoshihide,
Nakano Isao,
Katano Yoshiaki,
Takayama Tetsuo,
Kumada Takashi,
Nakano Satoshi,
Takamatsu Junki,
Saito Hidehiko,
Hayakawa Tetsuo
Publication year - 1998
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199802)54:2<80::aid-jmv2>3.0.co;2-k
Subject(s) - viral quasispecies , virology , hepatitis c virus , virus , hypervariable region , hepacivirus , viral disease , flaviviridae , hepatitis c , polymerase chain reaction , biology , medicine , immunology , antibody , gene , genetics
The quasispecies nature of hepatitis C virus (HCV) in patients with mixed HCV subtype infection was compared with that in patients with single HCV subtype infection. The number of HCV quasispecies was compared between 35 patients with mixed HCV subtype infection and 83 patients with single subtype infection. Subtype was determined by primers deduced from the core region and by line probe assay respectively. The number of quasispecies was evaluated by polymerase chain reaction amplification of hypervariable region 1 and by fluorescence single‐strand conformation polymorphism analysis. There was no difference in clinical background between patients with mixed subtype infection and patients with single subtype infection. The number of quasispecies in patients with multiple subtype HCV infection was larger than in patients with single subtype HCV infection. The immunologic environments which allow the coexistence of more HCV quasispecies in patients with multiple HCV subtype infection differs from that in patients with single HCV subtype infection. J. Med. Virol. 54:80–85, 1998. © 1998 Wiley‐Liss,Inc.