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The detection of intrathecal synthesis of anti‐herpes simplex IgG antibodies: Comparison between an antigen‐mediated immunoblotting technique and antibody index calculations
Author(s) -
Monteyne Philippe,
Albert Frauke,
Weissbrich Benedikt,
Zardini Elisabetta,
Ciardi Maria,
Cleator Graham M.,
Sindic Christian J. M.
Publication year - 1997
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199712)53:4<324::aid-jmv3>3.0.co;2-9
Subject(s) - antibody , herpes simplex virus , virology , antigen , serology , cerebrospinal fluid , encephalitis , medicine , virus , immunology , alphaherpesvirinae , intrathecal , herpesviridae , multiple sclerosis , western blot , varicella zoster virus , viral disease , biology , pathology , surgery , biochemistry , gene
The detection of intrathecal antibody synthesis was compared by the calculation of antibody indices (AI) derived from ELISA techniques with the detection of virus‐specific oligoclonal IgGs by an antigen‐mediated capillary blot technique. Twenty‐seven paired serum and cerebrospinal fluid (CSF) samples were examined from 15 immunocompetent patients with herpes simplex virus encephalitis (HSE) diagnosed by PCR on early CSF samples. These techniques were also applied to paired samples from 20 multiple sclerosis (MS) patients, 10 patients with other inflammatory neurological diseases and 10 patients with non inflammatory neurological disorders. There was a good correlation between the results obtained by AI and those obtained by immunoblotting, especially in HSE (2 discordant results out of 27). Discrepancies were more frequent (25%) in MS patients where a “polyspecific” reaction characterized by low affinity antibodies is known to occur. Some of the discrepancies could, in part, be due to serological cross‐reaction with varicella zoster virus. J. Med. Virol. 53:324–331, 1997. © 1997 Wiley‐Liss, Inc.