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Detection rate and intratumoral virus load of human herpesvirus‐8 in immunodeficiency‐related B‐cell lymphoid malignancies
Author(s) -
Feuillard Jean,
Aubin JeanThierry,
Poirel Laurent,
Davi Frédéric,
Kujas Michèle,
Rousselet MarieChristine,
Angonin Régis,
Raphaël Martine,
Agut Henri
Publication year - 1997
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199711)53:3<277::aid-jmv17>3.0.co;2-e
Subject(s) - primary effusion lymphoma , lymphoma , viral load , virus , virology , gammaherpesvirinae , viral disease , lymphoproliferative disorders , herpesviridae , immunodeficiency , immunology , epstein–barr virus , biology , medicine , immune system
Human herpesvirus‐8 (HHV‐8), associated with Kaposi's sarcoma, primary effusion lymphoma, and Castleman's disease, has been found in circulating B‐cells and might have a causative role in B‐cell malignancies associated with immunodeficiency syndromes. We determined the rate of detection and intratumoral virus load of HHV‐8 by means of a semiquantitative approach in post‐transplant lymphoproliferative diseases (PTLDs), AIDS‐related non‐Hodgkin's lymphomas (NHLs), including both Burkitt's lymphomas (BLs) and large cell lymphomas (LCLs), as well as in control groups consisting of follicular hyperplasias (FHs) and HIV‐negative LCLs. HHV‐8 sequences were detected at a similar rate in HIV‐negative PTLDs (24%), HIV‐negative LCLs (22%) and HIV‐negative FHs (17%). The detection rate was significantly higher in HIV‐positive BLs (73%), HIV‐positive LCLs (67%), and HIV‐positive FHs (65%) supporting the view of an epidemiological link between HHV‐8 and HIV infections. The viral load was 10 2 genome copies per cell in the single case of primary effusion lymphoma included in the LCL group while it was 10 −3 copy per cell (median value; range: 10 −4 –10 −1 ) in all the other HHV‐8‐positive samples. No significant difference of viral load was found according to HIV status. The virus loads of PTLDs and HIV‐positive LCLs were significantly higher than those observed in HIV‐positive BLs and FHs, suggesting, to some extent, that the degree of immunodeficiency may influence HHV‐8 replication. However, with the exception of the single case of primary effusion lymphoma studied, the low intratumoral load of HHV‐8 strongly argues against a direct causative agent of the virus in the occurrence of PTLDs and AIDS‐related NHLs. J. Med. Virol. 53:277–281, 1997. © 1997 Wiley‐Liss, Inc.