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Detection of human herpesvirus 8 DNA sequences in peripheral blood mononuclear cells of children
Author(s) -
Kikuta Hideaki,
Itakura Osamu,
Ariga Tadashi,
Kobayashi Kunihiko
Publication year - 1997
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199709)53:1<81::aid-jmv14>3.0.co;2-#
Subject(s) - polymerase chain reaction , primer (cosmetics) , southern blot , peripheral blood mononuclear cell , biology , virology , microbiology and biotechnology , nested polymerase chain reaction , dna , population , genomic dna , real time polymerase chain reaction , gene , medicine , genetics , chemistry , environmental health , organic chemistry , in vitro
Human herpesvirus 8 (HHV‐8) DNA sequences were examined in peripheral blood mononuclear cell (PBMC) DNA samples of 56 children, 15 healthy adults, and 10 renal transplant patients by the polymerase chain reaction (PCR). The PCR amplification was carried out using the published KS330 233 primer pairs to amplify HHV‐8 DNA sequences. The PCR‐amplified products were confirmed by Southern blot hybridization with radiolabeled 233 bp HHV‐8 DNA fragment, which was cloned and sequenced from the PCR‐amplified product of Kaposi's sarcoma tissue. Six PCR‐amplified product of four children and two renal transplant patients were cloned and sequenced. HHV‐8 DNA sequences were detected in 36 of 56 (64%) normal children, in 12 of 15 (80%) healthy adults, and in all 10 renal transplant patients by Southern blot hybridization of PCR‐amplified products. Six PCR‐amplified products were confirmed by sequencing. These results suggest that HHV‐8 infection is prevalent in the Japanese population with infection occurring in early childhood. J. Med. Virol. 53:81–84, 1997. © 1997 Wiley‐Liss, Inc.