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RT‐PCR based diagnosis and molecular characterisation of mumps viruses derived from clinical specimens collected during the 1996 mumps outbreak in Portugal
Author(s) -
Afzal M. A.,
Buchanan J.,
Dias J. A.,
Cordeiro M.,
Bentley M. L.,
Shorrock C. A.,
Minor P. D.
Publication year - 1997
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199708)52:4<349::aid-jmv1>3.0.co;2-1
Subject(s) - mumps virus , virology , outbreak , biology , phylogenetic tree , polymerase chain reaction , virus , molecular epidemiology , paramyxoviridae , nucleic acid sequence , viral disease , genotype , genetics , gene
Clinical specimens collected during an outbreak of mumps were characterised by RT‐PCR, nested PCR, and nucleotide sequencing. Mumps virus was positively identified in 12/21(57%) saliva, 9/21(43%), throat and 1/33(3%) urine specimens and further sequence comparison revealed that at least six strains of viruses, which differed from 0–9.43% at the nucleotide levels, were co‐circulating during the epidemic. However, phylogenetic analysis showed that these viruses grouped with two previously identified lineages which were mostly composed of other European mumps virus isolates. J. Med. Virol. 52:349–353, 1997. © 1997 Wiley‐Liss, Inc.