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Detection of mumps virus genome directly from clinical samples and a simple method for genetic differentiation of the Hoshino vaccine strain from wild strains of mumps virus
Author(s) -
Kashiwagi Yasuyo,
Kawashima Hisashi,
Takekuma Kouji,
Hoshika Akinori,
Mori Takayuki,
Nakayama Tetsuo
Publication year - 1997
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199706)52:2<195::aid-jmv13>3.0.co;2-k
Subject(s) - virology , biology , mumps virus , virus , restriction fragment length polymorphism , genome , strain (injury) , polymerase chain reaction , mumps vaccine , microbiology and biotechnology , gene , vaccination , genetics , measles , anatomy , measles vaccine
A simple and sensitive method was developed for the differentiation of the Hoshino vaccine strain from wild strains with a restriction fragment length polymorphism (RFLP) analysis in the part of hemagglutinin‐neuraminidase (HN) gene. The virus genome was amplified by using a reverse transcriptase‐polymerase chain reaction (RT‐PCR) directly from clinical samples. The PCR product of the Hoshino vaccine strain was cleaved into 2 fragments after digestion with Sca I and Afl II. All wild strains showed 2 RFLP profiles, A and B, different from that of vaccine strain. Wild A strains were cut into 2 fragments after digestion with Sca I only, while wild B strains were cleaved neither with Sca I nor Afl II. This molecular approach provides an effective method for differentiation of the Hoshino vaccine strain from wild strains of mumps virus in patients after vaccination. J. Med. Virol. 52:195–199, 1997. © 1997 Wiley‐Liss, Inc.