Premium
Detection of GB Virus‐C/Hepatitis G Virus RNA in Serum by Reverse Transcription Polymerase Chain Reaction
Author(s) -
Bhardwaj Bhavna,
Qian KePing,
Detmer Jill,
Mizokami Masashi,
Kolberg Janice A.,
Urdea Mickey S.,
Schlauder George,
Linnen Jeffrey M.,
Kim Jungsuh P.,
Davis Gary L.,
Lau Johnson Y. N.
Publication year - 1997
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199705)52:1<92::aid-jmv15>3.0.co;2-2
Subject(s) - virology , gb virus c , ns3 , virus , hepatitis c virus , amplicon , reverse transcription polymerase chain reaction , polymerase chain reaction , reverse transcriptase , biology , untranslated region , rna , flaviviridae , microbiology and biotechnology , gene , messenger rna , genetics
Three PCR methods based on the GB virus‐C/hepatitis G virus (GBV‐C/HGV) 5′UTR and NS3 genomic region were used for the detection of GBV‐C/HBV RNA in serum of 62 patients with chronic hepatitis C virus (HCV) infection. Ten of 62 (16%) patients were found to have GBV‐C/HGV RNA, which was confirmed by sequence analysis of the 5′UTR PCR amplicon. All methods appear to be specific, but methods based on the 5′UTR appear to be more sensitive. J. Med. Virol. 52:92–96, 1997. © 1997 Wiley‐Liss, Inc.