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Assembly of JC virus‐like particles in COS7 cells
Author(s) -
Shishido Yukiko,
Nukuzuma Soichi,
Mukaigawa Jun,
Morikawa Shigeru,
Yasui Kotaro,
Nagashima Kazuo
Publication year - 1997
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199704)51:4<265::aid-jmv2>3.0.co;2-3
Subject(s) - capsid , transfection , virology , virus , biology , viral entry , cell culture , microbiology and biotechnology , viral replication , genetics
JC virus lacks an appropriate cell line to support virus replication. The establishment of a JC pseudovirus assembly system could play an alternative role for a virus culture system. COS7 cells and a transfer vector, pcDL‐SRα296, were used to express JC viral structural genes. VP231‐SRα, which encodes VP2/VP3 and VP1, but lacks 137 bp of the 5′‐terminus of agnogene, showed both efficient nuclear migration and quantitative expression of the major capsid protein VP1. JC pseudovirus assembly was observed in the nucleus of VP231‐SRα transfected cells. Evidence of JC pseudovirus assembly is presented. The further utilization of this system, which includes a study for the viral morphogenesis, serological diagnosis, as well as the potential application for gene transfer vector, is discussed. J. Med. Virol. 51:265–272, 1997. © 1997 Wiley‐Liss, Inc.