Premium
Rapid detection and serotyping of adenovirus by direct immunofluorescence
Author(s) -
Wood Samuel R.,
Sharp Ian R.,
Caul E. Owen,
Paul Ian,
Bailey Andrew S.,
Hawkins Michael,
Pugh Simon,
Treharne John,
Stevenson S.
Publication year - 1997
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199703)51:3<198::aid-jmv9>3.0.co;2-1
Subject(s) - monoclonal antibody , virology , serotype , antibody , typing , biology , immunofluorescence , adenoviridae , antigen , mastadenovirus , microbiology and biotechnology , recombinant dna , immunology , gene , genetics
Abstract Four fluorescent antibody reagents were evaluated for their suitability for the identification of adenovirus isolates by immunofluorescence. The antibodies used in the reagents consist of monoclonal antibodies against adenovirus type 3 (Ad3), Ad4, Ad8, and adenoviruses of subgroup C (Ad1,2,5,6), serotypes known to occur in outbreaks of disease. Most of the monoclonal antibodies employed were reactive against type‐specific antigens found on the hexon protein. Reagents employing two noncompeting anti‐hexon antibodies were more sensitive than reagents prepared with only one monoclonal antibody, although both types of reagents exhibited a high degree of specificity. Five hundred and seventeen adenovirus isolates (359 of which had previously been typed by other methods) and 46 nonadenovirus isolates were examined with all four type‐specific reagents in parallel with an adenovirus group‐specific reagent. The results indicate that direct typing of adenovirus isolates is feasible, leading to significant savings in time compared to other typing methods and should contribute to the management of certain adenovirus infections, particularly during outbreaks. J. Med. Virol. 51:198–201, 1997. © 1997 Wiley‐Liss, Inc.