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Sensitive detection of human Caliciviridae by RT‐PCR
Author(s) -
SteneJohansen Kathrine,
Grinde Bjorn
Publication year - 1996
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199611)50:3<207::aid-jmv1>3.0.co;2-d
Subject(s) - caliciviridae , biology , norwalk virus , virology , phylogenetic tree , polymerase chain reaction , virus , viral disease , gene , genetics , norovirus
A semi‐nested reverse transcriptase‐polymerase chain reaction (RT‐PCR) was developed for the detection of human Caliciviridae . The method was evaluated on faecal samples from patients with gastroenteritis sent to the Norwegian National Institute of Public Health for routine diagnosis by direct electron microscopy (EM). Of 166 samples, 49 were found to contain Caliciviridae by EM, while 7 samples contained other viruses. A total of 74 samples was positive by PCR, including all the samples with EM detectable Caliciviridae , while specimens containing other agents were negative. Phylogenetic analysis of RNA sequences from 14 Norwegian samples indicated that the viruses present in Norway are evenly distributed when compared to sequences of human Caliciviridae from other countries. The PCR primers should therefore be useful for samples from other regions. The phylogenetic analysis did not cluster viruses with a calici‐like morphology, but mingled them with sequences from Norwalk‐like viruses, indicating that the two morphological types do not represent separate genogroups. © 1996 Wiley‐Liss, Inc.