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Detection and sequence analysis of the major immediate early and PP150 gene of latent human cytomegalovirus in spleen, liver, and kidney tissues of trauma victims
Author(s) -
Hendrix M. G. Ron,
Beuken Eric,
Slobbe Rob L.,
Bruggeman Cathrien A.
Publication year - 1996
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199610)50:2<193::aid-jmv14>3.0.co;2-n
Subject(s) - virology , cytomegalovirus , gene , biology , sequence (biology) , spleen , human cytomegalovirus , gene sequence , sequence analysis , kidney , herpesviridae , virus , viral disease , immunology , genetics , phylogenetic tree
The presence of human cytomegalovirus (HCMV) DNA in liver, spleen, and kidney samples of HCMV‐seropositive trauma victims during latency was demonstrated by polymerase chain reaction (PCR), using primers reactive with the major immediate early gene exon 4 and the structural gene pp150. Sequence analysis of the PCR amplificates showed more than 95% homology with the reference HCMV strain AD169. The few mutations observed were mostly distributed randomly. In one subject two types of the MIE‐4 gene were detected, and in another subject two types of the pp150 gene were found, suggesting that different strains of HCMV can be found in organs of the same patient during latency. © 1996 Wiley‐Liss, Inc.