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Detection of human viruses using primary cells immortalised by oncogene transfection, in comparison with primary cells and established cell lines
Author(s) -
Clarke J. B.,
Chakraverty P.,
KreuzbergDuffy U.,
MacDonald C.,
Moulsdale H.,
Golding J.,
Peters J.,
Griffiths J. B.
Publication year - 1996
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199610)50:2<176::aid-jmv11>3.0.co;2-k
Subject(s) - primary cell , transfection , cell culture , virology , biology , hek 293 cells , virus , immortalised cell line , microbiology and biotechnology , genetics
No single established cell line was found capable of substituting for primary baboon kidney (PBK) or primary rhesus macacque kidney (PRK) cells for detection of human viruses. Although a panel of cell lines could detect influenza, parainfluenza, and enteroviruses, which are among the most important viruses encountered in routine diagnostic laboratories, the sensitivity of this panel was not as high as that of PBK or PRK cells. However, in a promising complementary approach, PBK and PRK cells have been immortalised successfully by oncogene transfection, and some of the resulting cell lines have retained susceptibility to human viruses, and may be suitable for routine diagnostic use. © 1996 Wiley‐Liss, Inc.