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Humoral immunity patterns based on antibody reactivity to rotavirus antigens in Brazilian children under 5 years of age
Author(s) -
Andrade Gildete P.,
Lima Lourdes R.A.V.,
HoshinoShimizu Sumie,
Oliveira Maria I.,
Mendonça Ronaldo Z.,
Hársi Charlotte M.,
Stewien Klaus E.
Publication year - 1996
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199607)49:3<212::aid-jmv9>3.0.co;2-7
Subject(s) - antibody , rotavirus , serology , virology , immunity , antigen , immunology , humoral immunity , biology , reoviridae , immune system , hemagglutination assay , immunoassay , medicine , virus , titer
The age distribution of antibody to simian rotavirus (SA‐11) was studied in serum specimens obtained from 399 children aged to 5 years and living in the city of Recife (PE), located in the north eastern region of Brazil. Sera were examined for group‐specific rotavirus antibody using a blocking enzyme immunoassay (bELISA) and a hemagglutination inhibition antibody (HIA) test, and for anti‐VP2, anti‐VP4, anti‐VP6, and anti‐VP7 antibodies using an immunoblotting assay (IBA). Antibody prevalence was similar in all bELISA and HIA assays, showing a steep rise in the 6‐ to 17‐month‐old age groups. The results indicate early acquisition of antibody to rotavirus. The majority of children aged 2 to 4 years had bELISA (50% to 60%) and HIA (70% to 81%) antibodies. There was an association in prevalence data obtained by HIA and bELISA with immunoblotting (IBA), revealing four serologic profiles. Children with profiles I and II (60%) respectively had HAI and ELISA antibody or HAI antibody alone and all had immunoprotective antibodies to VP4 and/or VP7. These children were regarded as “immune,” resembling convalescent patients with a rotavirus infection. Children with profile III (4%) had no HIA antibody and only non‐protective anti‐VP6 and/or VP7 antibody, and were considered to be “partially immune.” Children with profile IV (36%) had no detectable antibody and were classified as “nonimmune.” These children should be considered to be susceptible to rotavirus infection, with the risk of developing clinically severe diarrhea. © 1996 Wiley‐Liss, Inc.