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Determination of the inactivation kinetics of hepatitis A virus in human plasma products using a simple TCID 50 assay
Author(s) -
Barrett P.N.,
Meyer H.,
Wachtel I.,
Eibl J.,
Dorner F.
Publication year - 1996
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199605)49:1<1::aid-jmv1>3.0.co;2-a
Subject(s) - hepatitis a virus , titration , virology , virus , cytopathic effect , virus quantification , virus inactivation , chemistry , aqueous solution , strain (injury) , microbiology and biotechnology , biology , organic chemistry , anatomy
The transmission of hepatitis A virus (HAV) associated with use of FVIII concentrates has been reported in a number of European countries. All of these cases were associated with products inactivated by use of solvent detergent treatment. These reports have emphasized the necessity of evaluating virus inactivation methodologies for their ability to inactivate HAV. Such studies had previously been hampered by the difficulties associated with titration of HAV, because of the minimal cytopathic effect of most strains of virus on tissue culture cells. We have developed a simple, rapid, TCID 50 virus titration system using a cytopathic strain of HAV which allows extensive kinetic studies of HAV inactivation. This has been compared with the standard radioimmunofocus forming (RFF) assay which is presently used for HAV titration. The reproducibility of the TCID 50 assay was demonstrated to be equal to that of the RFF assay and the 95% confidence intervals for titres determined by both assays were also equal. The thermal stability of the cytopathic strain was studied and shown to be equivalent to that of a noncytopathic strain. The kinetics of HAV inactivation by heating in aqueous solution were compared to those of HIV‐1 and a number of model viruses. It was demonstrated that HAV was highly stable, with 5 hours heat treatment at 60°C in aqueous solution being required to inactivate 5.8 log 10 virus. In contrast to heating in aqueous solution, lyophilization followed by 1 hour vapor heating at 60°C was sufficient to inactivate 5.9 log 10 HAV. © 1996 Wiley‐Liss, Inc.