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Analysis of the human serological immune response to a variable region of the attachment (G) protein of respiratory syncytial virus during primary infection
Author(s) -
Cane Patricia A.,
Thomas Huw M.,
Simpson Anna F.,
Evans Johanna E.,
Hart Charles A.,
Pringle Craig R.
Publication year - 1996
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199603)48:3<253::aid-jmv7>3.0.co;2-7
Subject(s) - virology , serology , virus , biology , antibody , fusion protein , mononegavirales , paramyxoviridae , genotype , immune system , immunology , viral disease , gene , recombinant dna , genetics
The serum antibody responses of babies to the variable carboxy‐terminal region of the attachment (G) protein of respiratory syncytial virus (RSV) have been analysed using paired acute and convalescent sera from infants experiencing their first RSV infection with viruses of known genotype. The variable 84–85 carboxy‐terminal amino acids of the G protein of six recent isolates of group A RSV were expressed in Escherichia coli as fusion proteins with glutathione S‐transferase. About half the infants developed antibodies which recognised these fusion proteins. The patterns of response obtained in enzyme linked immunosorbant assays and immunoblotting assays were closely related to the infecting genotype. © 1996 Wiley‐Liss, Inc.