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Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines
Author(s) -
Tsuboi So,
Nagamori Seishi,
Miyazaki Masahiro,
Mihara Koichiro,
Fukaya Kenichi,
Teruya Kiichiro,
Kosaka Tsunenori,
Tsuji Takao,
Namba Masayoshi
Publication year - 1996
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199602)48:2<133::aid-jmv3>3.0.co;2-a
Subject(s) - virology , hepatocellular carcinoma , hepatitis c virus , persistence (discontinuity) , cell culture , virus , biology , hepatitis a virus , rna , gene , cancer research , genetics , geotechnical engineering , engineering
The persistence of the viral RNA of hepatitis C virus (HCV) was examined in 13 hepatocellular carcinoma (HCC) and two hepatoblastoma cell lines by reverse transcription followed by the polymerase chain reaction (RT‐PCR). HCV RNA was detected in three HCC lines (JHH‐1, JHH‐4, and JHH‐6) and negative‐strand viral RNA was found in JHH‐4, indicating that there is a putative replicative intermediate of HCV in JHH‐4 cells. To rule out the possibility of contamination, the partial nucleotide sequences of HCV‐specific PCR products of these three cell lines were determined. The clone from JHH‐1 belonged to genotype 1 (1a or 1b), and the clones from JHH‐4 and JHH‐6 belonged to genotype 2b, but their sequences differed from each other. These cell lines may be useful for studies related to HCV. © 1996 Wiley‐Liss, Inc.