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Detection of BCR/ABL gene rearrangement and the elimination of rearranged clone in chronic myelocytic leukemia patients
Author(s) -
Eren E.,
Aytac U.,
Tetik E.,
Akman O.,
Kansu E.,
Gunduz U.
Publication year - 2000
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/(sici)1096-8652(200002)63:2<85::aid-ajh5>3.0.co;2-l
Subject(s) - clone (java method) , breakpoint cluster region , southern blot , chromosomal translocation , microbiology and biotechnology , gene rearrangement , myelocytic leukemia , biology , philadelphia chromosome , abl , gene , polymerase chain reaction , leukemia , k562 cells , virology , immunology , genetics , tyrosine kinase , receptor
Cytogenetic and molecular studies were performed on 20 interferon‐α receiving Turkish chronic myelocytic leukemia patients. Four different restriction endonucleases and bcr‐G probe were used for southern blot analysis to detect rearrangements of the bcr gene. The RT‐PCR method was also applied to detect chimeric bcr/abl mRNA. Seventeen patients showed a chromosomal break within the 5.8 kb M‐bcr region by southern blot analysis while three cases out of 20 have not shown any rearrangement. These three cases were further analysed by RT‐PCR and they were also found to be carrying the Philadelphia translocation (Ph). However, in four years of follow‐up this RT‐PCR positivity has disappeared, which suggests an elimination of Ph clone with prolonged interferon‐α treatment. Am. J. Hematol. 63:85–89, 2000. © 2000 Wiley‐Liss, Inc.