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Persistent infection of a lymphoma cell line by herpes simplex virus
Author(s) -
Kao Yuan S.,
Sundin Daniel R.,
Gebhardt Bryan M.
Publication year - 1999
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/(sici)1096-8652(199910)62:2<93::aid-ajh5>3.0.co;2-7
Subject(s) - herpes simplex virus , virology , biology , virus , herpesviridae , cell culture , lymphoma , simplexvirus , lymphoblast , viral disease , immunology , genetics
The peripheral blood cells from a patient with a B‐cell lymphoma were established in long‐term tissue culture. Two years after establishment of the cells in culture they were infected with herpes simplex virus type 2 and the productivity and duration of viral persistence investigated. One week after infection the lymphoblastoid cells were productively infected and have remained so for a period of over 3 years. Expression of a viral glycoprotein antigen was evaluated by using a fluorescein‐labeled monoclonal anti‐herpes simplex virus type 2 antibody and revealed a spectrum of staining reactions grading from a lightly stippled to very intense pattern. Polymerase chain reaction analysis of the infected cells revealed the presence of the herpes simplex virus type 2 DNA polymerase gene in the infected cells that was absent from the uninfected lymphoblastoid cells. These results taken together with the long‐term growth characteristics of both the infected and uninfected lymphoblastoid cells suggest that this cell line may be a good model system for studying viral infection, viral replication, viral latency, and clinical application for the isolation of human herpes virus. Am. J. Hematol. 62:93–98, 1999. © 1999 Wiley‐Liss, Inc.