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Abnormal proliferation of CD4 − CD8 + γδ + T cells with chromosome 6 anomaly: Role of fas ligand expression in spontaneous regression of the cells
Author(s) -
Ichikawa Naoaki,
Kitano Kiyoshi,
Ito Toshiro,
Nakazawa Takayuki,
Shimodaira Shigetaka,
Ishida Fumihiro,
Kiyosawa Kendo
Publication year - 1999
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/(sici)1096-8652(199904)60:4<305::aid-ajh9>3.0.co;2-6
Subject(s) - cd8 , cd3 , microbiology and biotechnology , lymphocyte , biology , fas ligand , t cell , cytotoxic t cell , immunology , apoptosis , immune system , programmed cell death , genetics , in vitro
We report a case of granular lymphocyte proliferative disorder accompanied with hemolytic anemia and neutropenia. Phenotypes of the cells were T cell receptor γδ + CD3 + CD4 − CD8 + CD16 + CD56 − CD57 − . Southern blot analysis of T cell receptor β and γ chains demonstrated rearranged bands in both. Chromosomal analysis after IL‐2 stimulation showed deletion of chromosome 6. Sorted γδ + T cells showed an increase in Fas ligand expression compared with the levels in sorted αβ + T cells. The expression of Fas ligand on these γδ + T cells increased after IL‐2 stimulation. The patient's anemia improved along with a decrease in granular lymphocyte count and disappearance of the abnormal karyotype without treatment. The expression of Fas ligand may be involved in spontaneous regression of granular lymphocyte proliferation with hemolytic anemia. Am. J. Hematol. 60:305–308, 1999. © 1999 Wiley‐Liss, Inc.