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Longitudinal analysis of circulating myeloma cells detected by allele specific mRNA in situ hybridization
Author(s) -
Brown Ross D.,
Luo XiaoFeng,
Gibson John,
Brisco Michael,
Sykes Pam,
Morley Alec,
Joshua Doug
Publication year - 1998
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/(sici)1096-8652(199808)58:4<273::aid-ajh4>3.0.co;2-n
Subject(s) - peripheral blood mononuclear cell , multiple myeloma , biology , in situ hybridization , antibody , microbiology and biotechnology , population , circulating tumor cell , pathology , cancer research , immunology , medicine , messenger rna , gene , cancer , genetics , metastasis , in vitro , environmental health
Individual myeloma cells in the peripheral blood of 7 patients with multiple myeloma were detected by mRNA in situ hybridization (ISH) using biotinylated antisense oligonucleotide probes to non‐germline sequences of the CDR3 region of the immunoglobulin heavy chain gene. Peripheral blood samples from these patients were studied over a period of 2–3 years. The number of circulating myeloma cells varied from 0.1–23% of the mononuclear cell population. Longitudinal studies showed that the highest number of circulating myeloma cells were present during escape phase and thus the percentage of mRNA ISH+ cells correlated with the clinical state of the patient. This technique is the most accurate means of monitoring and quantitating individual myeloma cells in the peripheral blood of patients with myeloma and provides insight into the relevance of circulating myeloma cells. Am. J. Hematol. 58:273–277, 1998. © 1998 Wiley‐Liss, Inc.