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Two distinct truncated variants of ankyrin associated with hereditary spherocytosis
Author(s) -
Hayette S.,
Carré G.,
Bozon M.,
Alloisio N.,
Maillet P.,
Wilmotte R.,
Pascal O.,
Reynaud J.,
Reman O.,
Stéphan J.L.,
Morlé L.,
Delaunay J.
Publication year - 1998
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/(sici)1096-8652(199805)58:1<36::aid-ajh7>3.0.co;2-1
Subject(s) - hereditary spherocytosis , ankyrin , nonsense , microbiology and biotechnology , ankyrin repeat , genetics , biology , spherocytosis , nonsense mutation , exon , mutation , missense mutation , gene , immunology , splenectomy , spleen
We present two distinct truncated variants of ankyrin associated with mild to moderate hereditary spherocytosis. Ankyrin Saint‐Etienne 1 was manifested by an additional band located between bands 2.1 and 2.2. It was associated with a nonsense mutation in exon 39: TGG→TGA; W1721X. Ankyrin Saint‐Etienne 2 appeared as two faint bands underlining bands 2.1 and 2.2. It was associated with a nonsense mutation in exon 41: CGA→TGA; R1833X. Overall ankyrin was diminished in splenectomized patients. Messenger RNAs Saint‐Etienne 1 and 2 amounted to 20 and 37% of the total ankyrin mRNA, respectively. Ankyrin molecules truncated in their C‐terminal region retain some ability to bind to the membrane whereas the bulk of nonsense mutations, located in more upstream regions, result in the mere disappearance of one haploid set of ankyrin. In the present cases, it was not possible to apportion the roles of ankyrin reduction and truncation in the pathogenesis of hereditary spherocytosis. Am. J. Hematol. 58:36–41, 1998. © 1998 Wiley‐Liss, Inc.