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Lymphoma with multi gene rearrangement on the level of immunoglobulin heavy chain, light chains, and T‐cell receptor β chain
Author(s) -
Klein Ami,
Zemer Ruth,
Manor Yosef,
Shapiro Hava,
Cordoba Mario,
Spivak Isabella,
Radnay Judith
Publication year - 1997
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/(sici)1096-8652(199712)56:4<219::aid-ajh4>3.0.co;2-#
Subject(s) - immunoglobulin light chain , immunoglobulin heavy chain , gene rearrangement , lymphoma , antibody , heavy chain , chain (unit) , gene , cancer research , biology , immunology , genetics , physics , astronomy
A unique case with diffuse mixed malignant lymphoma was investigated for gene rearrangement on the level of T‐cell receptor (TCR), heavy chain immunoglobulin (Ig), and both light chains. Cell phenotype was examined with immunofluorescence techniques using antibodies against surface immunoglobulins (Slg) and the kappa and lambda light chains. Monoclonal antibodies were used against CD3, CD4, CD5, CD8, CD10, CD19, CD22, HLA‐DR, and TdT. Gene rearrangement analysis for monoclonality determination was carried out with restricted DNA (EcoR I and Hind III) hybridized with one of the following 32 P‐labelled probes: T‐cell receptor (TCR |gb), immunoglobulin heavy chain (JH), k light chain, and |gl light chain. Phenotyping of the cell population from the excised lymph node (LN) revealed the presence of 66% B‐cells and 35% T‐cells. Most of the B cells (94%) expressed μ heavy chain only. Expression of both light chains was negligible (k = 7% and λ = 2%). Gene rearrangement, which indicates monoclonality, was positive on the level of TCR, Ig heavy chain, and both light chains. The data obtained suggests a neoplastic transforming event in lymphoid stem cells, which preceded the subsequent differentiation process into either B or T lymphoma. Am. J. Hematol. 56:219–223, 1997. © 1997 Wiley‐Liss, Inc.