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Retinoblastoma protein expressed in human non‐Hodgkin's lymphoma cells generates resistance against radiation‐induced apoptosis
Author(s) -
Ishii Hideshi,
Igarashi Tadahiko,
Saito Toshiyuki,
Nakano Takashi,
Mori Masahiko,
Ohyama Harumi,
Miyamoto Tadaaki,
Saito Yasushi,
Oh Hakumei
Publication year - 1997
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/(sici)1096-8652(199705)55:1<46::aid-ajh9>3.0.co;2-3
Subject(s) - apoptosis , retinoblastoma , lymphoma , cancer research , retinoblastoma protein , cell culture , microbiology and biotechnology , biology , gene , tumor suppressor gene , oligonucleotide , cell cycle , immunology , carcinogenesis , genetics
Abstract Inactivation of the retinoblastoma susceptibility (Rb) gene has been observed in various cancers. Nevertheless, in several cancer cases, including non‐Hodgkin's lymphoma, previous investigations showed that the Rb gene product (pRb) was abundantly expressed with varying degree. Here we report the THS‐SP1.1 cell line isolated from non‐Hodgkin's lymphoma. The THS‐SP1.1 cells abundantly expressed pRb and showed resistance against radiation‐induced apoptosis. Culture with the antisense phosphorothioate oligonucleotide complementary to the Rb gene augmented the apoptosis of THS‐SP1.1 cells after radiation, whereas the control oligonucleotides did not. These data showed that pRb abundantly expressed in the lymphoma cells inhibited radiation‐induced apoptosis. Am J. Hematol. 55:46‐48, 1997. © 1997 Wiley‐Liss, Inc.