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Buffer may be the critical factor in measurement of anti‐prothrombin antibody on a γ‐ray‐irradiated plate by enzyme‐linked immunosorbent assay
Author(s) -
Matsuda Juzo,
Saitoh Noriko,
Tsukamoto Miyo,
Gotoh Moritaka,
Gohchi Kengo,
Kawasugi Kazuo
Publication year - 1996
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/(sici)1096-8652(199612)53:4<242::aid-ajh6>3.0.co;2-y
Subject(s) - phosphate buffered saline , buffer (optical fiber) , buffer solution , tris , enzyme , irradiation , antibody , chemistry , chromatography , saline , microbiology and biotechnology , biochemistry , medicine , immunology , biology , endocrinology , physics , telecommunications , computer science , nuclear physics
We investigated the influence of different buffers (Tris‐buffer and phosphate buffered saline (PBS)/Tween‐20 buffer) on anti‐prothrombin antibody (aPT) measurement by enzyme‐linked immunosorbent assay (ELISA), employing a γ‐ray‐irradiated plate. We found considerable discrepancies in aPT positivity between each buffer, and we suggest that the use of Tris‐buffer is not suitable for aPT measurement with a γ‐ray‐irradiated plate to measure aPT. © 1996 Wiley‐Liss, Inc.