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Expression of a cosmid containing the LCR, A γ, Δ, and β globin genes in mouse erythroleukemia cells
Author(s) -
Flamm Michael,
Bornschlegel Katherine,
Wu Shuang,
O'Neill David,
Bank Arthur
Publication year - 1996
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/(sici)1096-8652(199611)53:3<181::aid-ajh6>3.0.co;2-v
Subject(s) - cosmid , biology , gene , microbiology and biotechnology , clone (java method) , globin , locus control region , phenotype , transfection , gene expression , beta (programming language) , locus (genetics) , genetics , promoter , computer science , programming language
Abstract A novel method involving the packaging of phage particles was used to introduce cosmids containing components of the human β‐globin locus control region (LCR) and the human A γ, δ, and β globin genes into mouse erythroleukemia (MEL) cells. After stable transfection, MEL clones were selected and analyzed for expression of human genes. Both A γ and β globin mRNA were expressed in these clones, indicating that MEL cells do not suppress transcription of the human γ globin gene. The variability of human A γ vs. β globin expression from clone to clone prevents a clear delineation of differences in the expression of these two genes with a cosmid in which a region potentially involved in γ‐to‐β switching has been deleted. The results suggest that MEL cells are capable of supporting human γ expression, despite their predominantly adult phenotype. © 1996 Wiley‐Liss, Inc.