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Phospholipid composition and organization in model β‐thalassemic erythrocytes
Author(s) -
Kuypers Frans A.,
Schott Mary Ann,
Scott Mark D.
Publication year - 1996
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/(sici)1096-8652(199601)51:1<45::aid-ajh8>3.0.co;2-7
Subject(s) - phosphatidylethanolamine , phospholipid , phosphatidylcholine , phosphatidylserine , lysophosphatidylcholine , biophysics , chemistry , red blood cell , biochemistry , membrane , oxidative stress , membrane lipids , globin , hemoglobin , biology
The membrane phospholipid organization in human red blood cells (RBC) is rigidly maintained by a complex system of enzymes. However, several elements of this system are sensitive to oxidative damage. An important component in the destruction of β‐thalassemic RBC is the generation of reactive oxygen species and the release of redox‐active iron by the unpaired α‐hemoglobin chains. Consequently, we hypothesized that the presence of this oxidative stress to the RBC membrane could lead to alterations in membrane lipid organization. Model β thalassemic RBC, prepared by the introduction of excess α‐globin in the cell, have previously been shown to exhibit structural and functional changes almost identical to those observed in β‐thalassemic cells. After 24 hr at 37°C, the model β thalassemic cells exhibited a significant loss of deformability, as measured by ektacytometric analysis, indicative of extensive membrane damage. However, a normal steady‐state distribution of endogenous phospholipids was found, as evidenced by the accessibility of membrane phospholipids to hydrolysis by phospholipases. Similarly, the kinetics of transbilayer movement of spin‐labeled phosphatidylserine (PS) and phosphatidylethanolamine (PE) in all samples was in the normal range and was not affected by the presence of excess α‐globin chains. In contrast, a faster rate of spin‐labeled phosphatidylcholine (PC) transbilayer movement was observed in these cells. While control RBC exhibited a complete loss of their initial (2 mol%) lysophosphatidylcholine (LPC) levels following 24 hr of incubation at 37°C, 1.5 mol% LPC was still present in model β‐thalassemic cells, suggesting an altered phospholipid molecular species turnover, possibly as a result of an increased repair of oxidatively damaged phospholipids. © 1996 Wiley‐Liss, Inc.