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Improved DNA extraction from ancient bones using silica‐based spin columns
Author(s) -
Yang Dongya Y.,
Eng Barry,
Waye John S.,
Dudar J. Christopher,
Saunders Shelley R.
Publication year - 1998
Publication title -
american journal of physical anthropology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.146
H-Index - 119
eISSN - 1096-8644
pISSN - 0002-9483
DOI - 10.1002/(sici)1096-8644(199804)105:4<539::aid-ajpa10>3.0.co;2-1
Subject(s) - dna , dna extraction , extraction (chemistry) , polymerase chain reaction , chromatography , ancient dna , human bone , chemistry , materials science , microbiology and biotechnology , biology , biochemistry , gene , medicine , population , environmental health , in vitro
We describe a simple method for extracting polymerase chain reaction‐amplifiable DNA from ancient bones without the use of organic solvents. Bone powders are digested with proteinase K, and the DNA is purified directly using silica‐based spin columns (QIAquick™, QIAGEN). The efficiency of this protocol is demonstrated using human bone samples ranging in age from 15 to 5,000 years old. Am J Phys Anthropol 105:539–543, 1998. © 1998 Wiley‐Liss, Inc.