Polyglutamine expansion induced dynamic misfolding of androgen receptor

Abstract Spinal bulbar muscular atrophy (SBMA) is caused by a polyglutamine expansion (pQe) in the N‐terminal transactivation domain of the human androgen receptor (AR‐NTD), resulting in a combination of toxic gain‐ and loss‐of‐function mechanisms. The structural basis of these processes has not been resolved due to the disordered nature of the NTD, which hinders experimental analyses of its detailed conformations. Here, using extensive computational modeling, we show that AR‐NTD forms dynamic compact regions, which upon pQe re‐organize dynamically, mediated partly by direct pQ interaction with the Androgen N‐Terminal Signature (ANTS) motif. The altered dynamics of the NTD result in a perturbation of interdomain interactions, with potential implications for the binding of the receptor protein to its response element. Oligomeric aggregation of the dynamic misfolded NTD exposes pQe, but blocks tau‐5 and the FQNLF motif, which could lead to aberrant receptor transcriptional activity. These observations suggest a structural mechanism for AR dysfunction in SBMA.