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Milk Exosomes From Gestational Diabetes Mellitus Parturients Demonstrate Weaker Ability to Promote Intestinal Development in Offspring
Author(s) -
Mo Jiaqi,
Ding Yudi,
Yang Junyi,
Zheng Zhongdaixi,
Lu Jiazhi,
Luo Huiyu,
Wang Jiexian,
Lin Fengjuan,
Chen Junbin,
Li Qing,
Zheng Xiangyi,
Zha Longying
Publication year - 2025
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.70026
ABSTRACT This study aims to investigate whether human milk exosomes from gestational diabetes mellitus (GDM‐EXO) and healthy (HEA‐EXO) parturients differ in regulating intestinal development in offspring. The differential miRNAs associated with intestinal development in GDM‐EXO and HEA‐EXO were verified by using qPCR and their relationships with gut microbiota (GM) in infants were analyzed. C57BL/6J mice were gavaged with 50 mg/kg·BW HEA‐EXO or GDM‐EXO. The intestinal morphology, gut barriers, ZO‐1 and Occludin, and GM were determined by histological staining, Western blotting, and 16S rDNA amplicon sequencing, respectively. Hsa‐miR‐19b‐3p, hsa‐miR‐148a‐3p, and hsa‐miR‐320a‐3p were upregulated, and hsa‐miR‐429 was decreased in GDM‐EXO compared to HEA‐EXO. The GDM parturients’ infants had increased intestinal Coriobacteriaceae , Clostridiaceae , Erysipelotrichaceae , Erysipelatoclostridiaceae , and fewer Lactobacillaceae than the healthy parturient's infants. The four differential miRNAs in GDM‐EXO all correlated with the infants’ GM. GDM‐EXO‐ and HEA‐EXO‐fed mice had greater villus lengths, villus length‐to‐crypt depth ratios, goblet cell numbers, elevated ZO‐1 and Occludin, and lower crypt depths than control mice. HEA‐EXO‐fed mice had better intestinal morphology and gut barrier integrity than GDM‐EXO‐fed mice. GDM‐EXO‐fed mice had significantly decreased Lachnospiraceae and Oscillospiraceae than HEA‐EXO‐fed mice. GDM‐EXO demonstrate weaker ability to promote intestinal development in offspring than HEA‐EXO.
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