Pharmacokinetics of Oleracrylimide E in Rats Plasma Using UHPLC/‐ESI‐Q‐TOF/MS

ABSTRACT Oleracrylimide E, a new alkaloid from Portulaca oleracea L., shows significant bioactivity. Then, the pharmacokinetics of this alkaloid after its intravenous administration at dose of 2.0 mg/kg body weight in rat plasma was evaluated using UHPLC with vitexin‐2″‐O‐rhamnoside as an internal standard (IS). The UHPLC‐ESI‐Q‐TOF/MS method was applied to investigate the metabolism of Oleracrylimide E in rats in response to the immediate conversion of Oleracrylimide E into other metabolites after its oral administration. As a result, a total of 16 metabolites of Oleracrylimide E were identified in the plasma, urine, and fecal of the experimental rats.