RNF112 Facilitates Ubiquitin‐Mediated Degradation of c‐Myc, Suppressing Proliferation, Migration and Lipid Synthesis in Bladder Cancer | Zendy

Xiong Kangping | Zendy; Chen Siming | Zendy; Xu Huimin | Zendy; Tu Sheng | Zendy; Weng Hong | Zendy; Wang Yejinpeng | Zendy; Li Mingxing | Zendy; Yu Jingtian | Zendy; Qian Kaiyu | Zendy; Ju Lingao | Zendy; Zhang Yi | Zendy; Xiao Yu | Zendy; Wang Xinghuan | Zendy; Wang Gang | Zendy

Open Access

RNF112 Facilitates Ubiquitin‐Mediated Degradation of c‐Myc, Suppressing Proliferation, Migration and Lipid Synthesis in Bladder Cancer

Author(s) -

Xiong Kangping,

Chen Siming,

Xu Huimin,

Tu Sheng,

Weng Hong,

Wang Yejinpeng,

Li Mingxing,

Yu Jingtian,

Qian Kaiyu,

Ju Lingao,

Zhang Yi,

Xiao Yu,

Wang Xinghuan,

Wang Gang

Publication year - 2025

Publication title -

advanced science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.388

H-Index - 100

ISSN - 2198-3844

DOI - 10.1002/advs.202408311

Subject(s) - ubiquitin ligase , ubiquitin , cell growth , atp citrate lyase , lipid metabolism , chemistry , protein degradation , microbiology and biotechnology , biochemistry , enzyme , cancer research , biology , gene , citrate synthase

Abstract The E3 ubiquitin ligase RNF112 is significantly downregulated in bladder cancer (BLCA) and is correlated with disease progression. In vitro and in vivo studies indicated that RNF112 suppresses BLCA cell proliferation, migration, and lipid synthesis. Mechanistically, RNF112 directly interacts with the MB II domain of MYC through its N‐terminal zinc finger motif, and its catalytic site C97 facilitates K48‐linked polyubiquitination of the K389 residue on the c‐Myc protein, accelerating its degradation. Additionally, this research validated the interaction of c‐Myc with the promoter of ATP citrate lyase ( ACLY ), a central enzyme of lipid metabolism, promoting its transcriptional activity. The restoration of c‐Myc or ACLY expression attenuated the inhibitory effects of RNF112 on BLCA cell growth, migration, and lipid synthesis. In conclusion, this study confirmed that RNF112 suppressed the proliferation, migration, and lipid synthesis of BLCA cells by facilitating the ubiquitin‐mediated degradation of c‐Myc.

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