Mapping QTLs for Leafspot Resistance in Peanut Using SNP-Based Next-Generation Sequencing Markers | Zendy

Yuya Liang | Zendy; Michael R. Baring | Zendy; Shichen Wang | Zendy; Endang M. Septiningsih | Zendy

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Mapping QTLs for Leafspot Resistance in Peanut Using SNP-Based Next-Generation Sequencing Markers

Author(s) -

Yuya Liang,

Michael R. Baring,

Shichen Wang,

Endang M. Septiningsih

Publication year - 2017

Publication title -

plant breeding and biotechnology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.312

H-Index - 5

eISSN - 2287-9366

pISSN - 2287-9358

DOI - 10.9787/pbb.2017.5.2.115

Subject(s) - biology , snp , genetics , dna sequencing , resistance (ecology) , marker assisted selection , genetic marker , snp array , microsatellite , agronomy , genotype , single nucleotide polymorphism , gene , allele

Leafspot is one of the major diseases of peanut (Arachis hypogaea L.) that can cause more than 50% yield loss. The objective of this study was to identify and map quantitative trait loci (QTLs) for resistance to leafspot disease. An F2:6 recombinant inbred line (RIL) population, derived from a released cultivar Tamrun OL07 and a highly tolerant breeding line Tx964117, were used as a mapping population. A total of 90 RILs were planted for disease phenotyping in Yoakum, Texas in 2010 and 2012. A genetic map spanning the 20 linkage groups was developed using 1,211 SNP markers based on double digest restriction-site associated DNA sequencing (ddRAD-seq). A total of six quantitative trait loci (QTLs) were identified, with LOD score values of 3.2-5.0 and phenotypic variance explained ranging from 11%-24%. Major QTLs identified in this study may be used as potential targets for peanut improvement to leafspot disease through molecular breeding.

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