Expression analysis and Cloning of TaPase phosphatase gene in wheat (Triticum aestivum) | Zendy

Arun Sharma | Zendy

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Expression analysis and Cloning of TaPase phosphatase gene in wheat (Triticum aestivum)<br><br>

Author(s) -

Arun Sharma

Publication year - 2010

Publication title -

emirates journal of food and agriculture

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.304

H-Index - 28

eISSN - 2079-0538

pISSN - 2079-052X

DOI - 10.9755/ejfa.v22i6.4667

Subject(s) - abiotic stress , northern blot , gene , genomic dna , southern blot , cloning (programming) , biology , phosphatase , gene expression , abiotic component , microbiology and biotechnology , genetics , biochemistry , enzyme , paleontology , computer science , programming language

The present study was designed to clone gene encoding phosphatase and its regulation by abiotic stress treatments. The genomic DNA was isolated, TaPase DNA was amplified and cloned from wheat (Triticum aestivum). Expression studies by northern blot analysis was carried out by isolating RNA from tissues under different abiotic stress treatments. Clustal-W analysis of TaPase (assession no EU. 723832) with all the reported phosphatase genes revealed that codon encoding tryptophan (TGG) residue was conserved. Northern blot analysis revealed that under abiotic stress treatments like osmotic, salt and heat, the expression of TaPase was induced, indicating modulation of TaPase under stress treatments. Based upon these results, a possible physiological role of TaPase in wheat is discussed.

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