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Aims: Riboflavinis a B vitamin that is required for a wide variety of cellular processes. The absorbance spectrum of riboflavin was determined at different pH utilizing several buffers. The buffer at pH demon strating stable absorbance peaks with high numerical values of molar absorptivity is followed by accurate and sensitive assay of riboflavin by spectrophotometer. Study Design: The absorbance spectrum of riboflavin is determined in an aqueous buffer at various pH values. After identifying the absorbance peaks providing maximal molar absorptivity the assay of riboflavin in the identical buffer was undertaken.

Detection and Assay of Riboflavin (Vitamin B2) Utilizing UV/VIS Spectrophotometer and Citric Acid Buffer