Selection of Medium Components by Plackett-Burman Design for Cell Growth of a Newly Isolated Methylobacterium sp. WJ4 | Zendy

Wangjun Lee | Zendy; Jinwon Lee | Zendy

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Selection of Medium Components by Plackett-Burman Design for Cell Growth of a Newly Isolated Methylobacterium sp. WJ4

Author(s) -

Wangjun Lee,

Jinwon Lee

Publication year - 2016

Publication title -

korean chemical engineering research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.168

H-Index - 9

eISSN - 2233-9558

pISSN - 0304-128X

DOI - 10.9713/kcer.2016.54.6.812

Subject(s) - plackett–burman design , methylobacterium , bacteria , biology , chemistry , microbiology and biotechnology , 16s ribosomal rna , food science , genetics , fermentation

− We isolated a novel methylotrophic bacterium from soil collected in Dongducheon Environment Affairs Agency. The isolate was identified as Methylobacterium sp. WJ4 based on phylogenetic analysis. Plackett-Burman design was employed for screening eight parameters of nitrate mineral salts (NMS) medium for cell growth of a newly isolated Methylobacterium sp. WJ4 with experimental validation. Trace element solution and vitamin stock were found to affect cell growth, which can be further optimized for increased cell growth. This is the first report of screening parameters of NMS medium which affect cell growth of strain belonging to the genus Methylobacterium using PlackettBurman design.

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