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In this study, the effects of citrate buffer pretreatment conditions (solid-to-liquid ratio, reaction temperature, pH and concentration of buffer) on enzymatic hydrolysis of E. intestinalis for total reducing sugar (TRS) production were investigated. As a results of the citrate buffer pretreatment, a 5.40% hydrolysis yield was obtained under conditions including 1:10 solid-to-liquid ratio, 0.25 M citrate buffer (pH 3.5) at for 60 min. The maximum hydrolysis yield of 18.68% was obtained to enzymatic hydrolysis after pretreatment. This result is 1.81 times higher than that of control.

Production of Total Reducing Sugar from Enteromorpha intestinalis Using Citrate Buffer Pretreatment and Subsequent Enzymatic Hydrolysis