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Production of Total Reducing Sugar from Enteromorpha intestinalis Using Citrate Buffer Pretreatment and Subsequent Enzymatic Hydrolysis
Author(s) -
Donghyun Kim,
A-Ram Kim,
Don-Hee Park,
GwiTaek Jeong
Publication year - 2016
Publication title -
korean chemical engineering research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.168
H-Index - 9
eISSN - 2233-9558
pISSN - 0304-128X
DOI - 10.9713/kcer.2015.54.1.70
Subject(s) - sugar , enzymatic hydrolysis , hydrolysis , chemistry , phosphate buffered saline , buffer (optical fiber) , enzyme , food science , reducing sugar , biochemistry , biology , chromatography , computer science , telecommunications
In this study, the effects of citrate buffer pretreatment conditions (solid-to-liquid ratio, reaction temperature, pH and concentration of buffer) on enzymatic hydrolysis of E. intestinalis for total reducing sugar (TRS) production were investigated. As a results of the citrate buffer pretreatment, a 5.40% hydrolysis yield was obtained under conditions including 1:10 solid-to-liquid ratio, 0.25 M citrate buffer (pH 3.5) at for 60 min. The maximum hydrolysis yield of 18.68% was obtained to enzymatic hydrolysis after pretreatment. This result is 1.81 times higher than that of control.

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