Extraction of Genistein from Sophora flavescens with Supercritical Carbon Dioxide
Author(s) -
Chang-Nam Han,
Choon-Hyoung Kang
Publication year - 2015
Publication title -
korean chemical engineering research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.168
H-Index - 9
eISSN - 2233-9558
pISSN - 0304-128X
DOI - 10.9713/kcer.2015.53.4.445
Subject(s) - supercritical carbon dioxide , genistein , extraction (chemistry) , methanol , solvent , sophora flavescens , dpph , chromatography , chemistry , supercritical fluid extraction , bar (unit) , supercritical fluid , ethanol , carbon dioxide , aqueous solution , nuclear chemistry , antioxidant , organic chemistry , medicine , matrine , physics , meteorology
This study was directed to finding an optimum extraction condition of genistein from the S. flavescens with supercritical carbon dioxide as a solvent. In this effort, effects of the extraction conditions including pressure, tempera- ture and a co-solvent on the extraction efficiency were investigated. The aqueous ethanol and methanol solutions were used as co-solvents while the tested operating pressure and temperature ranges were from 200 bar to 300 bar and from 308.15 K to 323.15 K, respectively. The concentration of genistein was determined by means of HPLC equipped with a UV detector. From the results, it was observed that an increase in pressure led to the higher extraction efficiency. Further, methanol showed better performance as a co-slovent than ethanol. The DPPH radical scavenging activities were mea- sured to compare antioxidant activities of S. flavescens extracts.
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