OPTIMIZATION OF CALLUS INDUCTION AND SUBCULTURE CONDITIONS OF TAXUS CHINENSIS

Taxus chinensis (Pilger) Rehd.var. mairei (Lemee et Levl.) Cheng et L. K. Fu is an evergreen plant, which belongs to the Taxaceae family. These slow growing gymnosperms were harvested in large amounts to provide taxol for clinical trials. Due to the relative scarcity of the natural resources and the low yield of Paclitaxel (taxol), it is very necessary to produce alternate sources of taxol by callus cultures. In this work, we established conditions for callus induction and subculture of T. chinensis . The experimental results showed that B5 medium supplemented with 1 mg/L 2,4 - dichlorophenoxy acetic acid (2,4 D), 30 g/L sucrose and 7.5 g/L agar (pH 5.5) was effective for inducing callus and the better explants for callus induction was petiole. When the callus was sub cultured on seven different media and tamed several times, it could grow fast and anti - browning on the B5 medium containin g 1 mg/L α - naphthaleneacetic acid (NAA), 0.5 mg/L 2, 4 - D, 0.05 mg/L kinetin (KT), 0.05 g/L ascorbic acid (Vc), 0.1 g/L activated carbon (Ac), 30 g/L sucrose and 7.5 g/L agar (pH 5. 75).