IN VIVO AND IN VITRO RAPD ANALYSIS IN APOCYNACEAE FAMILY: POPULATION GROWING IN DIFFERENT REGIONS OF RAJASTHAN, INDIA

The present investigation included five samples hence, PCR - based DNA fingerprinting namely RAPD analysis was done to establish the relationship between the selected species of Rajasthan , India and in vivo and in vitro comparison of the same species. The RAPD analysis was conducted after isolation and purification the DNA of the all sample. In the present studies, the in vivo accessions of both plant samples 1, 2, 3, 5 and 2, 3, 4, 5 were collected from different geographical locat ions of Rajasthan, India which tend to accumulate genetic variations during the course of environmental adaptations. This Genetic diversity is also of great importance to the sustainability of plant population as there is a possibility of free or random po llen flow and fertilization. The identification of desert plants is crucial for their preservation and sustainable use, as well as to prevent forgery in the marketing of medici nal plants. Expected chemicals in the herbal plant targeted for medicinal use co uld vary with the genomic or environmental variability of the species.