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Nanotechnology is emerging as a new interdisciplinary field combining microbiology, Chemistry, physics, and material science. Recent advances promise developments in the synthesis, modification and practical applications of nanoparticles (NPs). Nanoparticles were excellent antibacterial agents with potential clinical applications. Sliver Nanoparticles have been successfully used in a wide range of applications including wound dressing, protective clothing, antibacterial surfaces, food preservation, and cosmetics as biocidal and disinfecting agents. The aim of this study was to investigate the mechanism of silver nanoparticle action against of methicillin-resistant Staphylococcus aureus. The Gram-positive methicillin-resistant Staphylococcus areas were used to evaluate the antibacterial activities of silver nanoparticles (Ag NPs). The growth rate of methicillin-resistant Staphylococcus aureus was investigated under varying Ag NPs concentrations by scanning electron microscope (SEM) & acridine orange /ethidium bromide (AO-EtBr) staining. The Quantitative Real-time PCR experiment showed that the mecA gene from the bacterial cells treated with Ag-NPs was downregulated compared to that in the untreated cells.

ATTENUATION OF GROWTH OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS IN RESPONSE TO SILVER NANOPARTICLES