Cellular Responses of Salmonella typhimurium Exposed to Green Tea Polyphenols | Zendy

Hyo-Kyung Choi | Zendy; KyeHeon Oh | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Cellular Responses of Salmonella typhimurium Exposed to Green Tea Polyphenols

Author(s) -

Hyo-Kyung Choi,

KyeHeon Oh

Publication year - 2012

Publication title -

the korean journal of microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.168

H-Index - 10

eISSN - 2383-9902

pISSN - 0440-2413

DOI - 10.7845/kjm.2012.48.2.087

Subject(s) - salmonella , polyphenol , peptide mass fingerprinting , camellia sinensis , biochemistry , gel electrophoresis , chemistry , peptide , polyacrylamide gel electrophoresis , biology , bacteria , enzyme , proteomics , gene , antioxidant , botany , genetics

The purpose of this study was to examine the cellular response of Salmonella typhimurium exposed to tea polyphenols (TPP) extracted from Korean green tea (Camellia sinensis L.). TPP showed a dose-dependent bactericidal effect on S. typhimurium. Analysis of cell membrane fatty acids of S. typhimurium cultures treated with TPP identified unique changes in saturated and unsaturated fatty acids, while scanning electron microscopic analysis demonstrated the presence of perforations and irregular rod forms with wrinkled surfaces in cells treated with TPP. Two-dimensional polyacrylamide gel electrophoresis of soluble protein fractions from S. typhimurium cultures showed 16 protein spots increased by TPP. These up-regulated proteins including proteins involved in antioxidants and chaperons, transcript and binding proteins, energy and DNA metabolism were identified by peptide mass fingerprinting using MALDI-TOF. These results provide clues for understanding the mechanism of TPP induced stress and cytotoxicity on S. typhimurium.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research