Viability of unstimulated lymphocytes exposed to extremely low frequency electromagnetic fields is dependent on intensity | Zendy

P Rajendra | Zendy; HN Sujatha | Zendy; R.B. Sashidhar | Zendy; Chakrapani Subramanyam | Zendy; D. Devendranath | Zendy; Rashmi Aradhya | Zendy

Open Access

Viability of unstimulated lymphocytes exposed to extremely low frequency electromagnetic fields is dependent on intensity

Author(s) -

P Rajendra,

HN Sujatha,

R.B. Sashidhar,

Chakrapani Subramanyam,

D. Devendranath,

Rashmi Aradhya

Publication year - 2012

Publication title -

biodiscovery

Language(s) - English

Resource type - Journals

ISSN - 2050-2966

DOI - 10.7750/biodiscovery.2012.2.2

Subject(s) - dna laddering , viability assay , apoptosis , flow cytometry , microbiology and biotechnology , intensity (physics) , electromagnetic field , mtt assay , chemistry , biology , andrology , medicine , biochemistry , programmed cell death , dna fragmentation , physics , optics , quantum mechanics

The cell viability and DNA damage in unstimulated sheep primary lymphocytes subjected to different extremely low electromagnetic field intensities (5, 50 and 100 µT; 50 Hz) were studied with special emphasis on apoptosis. Sheep primary lymphocytes cultured in RPMI, supplemented with 10% FBS in the absence of mitogens, were exposed till 16 h. The cell viability assessment by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay showed a dose dependent enhancement of viability at 16 h. Further, quantitative DNA laddering and flow cytometric analysis showed a significant decrease in apoptosis of the cells subjected to 100 (

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