Purification, characterization and antioxidant activities in vitro of polysaccharides from Amaranthus hybridus L. | Zendy

Zizhong Tang | Zendy; Caixia Zhou | Zendy; Yi Cai | Zendy; Yujia Tang | Zendy; Wenjun Sun | Zendy; Huipeng Yao | Zendy; Tianrun Zheng | Zendy; Hui Chen | Zendy; Yirong Xiao | Zendy; Zhi Shan | Zendy; Tongliang Bu | Zendy; Xiaoli Wang | Zendy; Lin Huang | Zendy; Lin Gou | Zendy

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Purification, characterization and antioxidant activities in vitro of polysaccharides from Amaranthus hybridus L.

Author(s) -

Zizhong Tang,

Caixia Zhou,

Yi Cai,

Yujia Tang,

Wenjun Sun,

Huipeng Yao,

Tianrun Zheng,

Hui Chen,

Yirong Xiao,

Zhi Shan,

Tongliang Bu,

Xiaoli Wang,

Lin Huang,

Lin Gou

Publication year - 2020

Publication title -

peerj

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.927

H-Index - 70

ISSN - 2167-8359

DOI - 10.7717/peerj.9077

Subject(s) - antioxidant , dpph , chemistry , amaranthus hybridus , food science , analytic hierarchy process , biochemistry , chromatography , traditional medicine , botany , biology , mathematics , medicine , operations research , weed

Background Amaranthus hybridus L. is an annual, erect or less commonly ascending herb that is a member of the Amaranthaceae family. Polysaccharides extracted from traditional Chinese medicines may be effective substances with antioxidant activity. Methods In this study, we isolated crude polysaccharides from A. hybridus (AHP-M) using microwave-assisted extraction. Then, the AHP-M was purified by chromatography with DEAE-32 cellulose, and two fractions, AHP-M-1 and AHP-M-2, were obtained. The structural characteristics of AHP-M-1 and AHP-M-2 were investigated, and their antioxidant activities were analyzed in vitro. Results We found that the monosaccharide composition of AHP-M-1 was different from that of AHP-M-2. The molecular weights of AHP-M-1 and AHP-M-2 were 77.625 kDa and 93.325 kDa, respectively. The results showed that the antioxidant activity of AHP-M-2 was better than that of AHP-M-1. For AHP-M-2, the DPPH radical scavenging rate at a concentration of 2 mg/mL was 78.87%, the hydroxyl radical scavenging rate was 39.34%, the superoxide anion radical scavenging rate was 80.2%, and the reduction ability of Fe 3+ was approximately 0.90. The total antioxidant capacity per milligram of AHP-M-2 was 6.42, which was higher than that of Vitamin C (Vc). Conclusion The in vitro test indicated that AHP-M-1 and AHP-M-2 have good antioxidant activity, demonstrating that A. hybridus L. polysaccharide has immense potential as a natural antioxidants.

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