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Bacterial inactivation is a crucial aspect of sanitation and hygiene. The effectiveness of slightly acidic electrolyzed water (SAEW) for reduction or removal of  Escherichia coli ,  Pseudomonas aeruginosa  and  Staphylococcus epidermidis  was evaluated. The bactericidal activity of SAEW and sodium hypochlorite (NaOCl) against  E. coli  and  P. aeruginosa  were compared through in vitro experiments. The effectiveness of SAEW spray was tested against  S. epidermidis . Results showed that SAEW had a more powerful bactericidal activity than NaOCl at the same available chlorine concentrations. For  E. coli , SAEW decreased the bacterial counts from 8.4 log 10  CFU/mL to less than 3.9 log 10  CFU/mL; NaOCl with the same available chlorine of 0.5 mg/L, caused a decrease from 8.4 log 10  CFU/mL to 7.1 log 10  CFU/mL. For  P. aeruginosa , SAEW caused bacterial counts to decrease from 8.5 log 10  CFU/mL to less than 4.1 log 10  CFU/mL against 8.5 log 10  CFU/mL to 6.2 log 10  CFU/mL for NaOCl with the same available chlorine of 0.5 mg/L. Spray experiments showed that 10 mg/L of SAEW spray decreased the bacterial counts of  S. epidermidis  from 3.7 log 10  CFU/m 3  to 2.8 log 10  CFU/m 3 , with 20 mg/L causing a reduction from 3.8 log 10  CFU/m 3  to 0 CFU/m 3 . The overall findings of this study indicate that SAEW may be a promising disinfectant agent either as a solution or spray.

Effectiveness of slightly acidic electrolyzed water on bacteria reduction: in vitro and spray evaluation