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Background  Aminopeptidases play important roles in various biological processes in nematodes including growth, development and reproduction. Although the aminopeptidases have been shown to regulate reproduction in  Caenorhabditis elegans  ( C. elegans ), the role of aminopeptidases in development and aging has not been reported. This study focused on the function of aminopeptidase AlaNyl aminopeptidase 1 (ANP-1) on development in  C. elegans .    Methods  In the present study, we reported the identification of ANP-1 in  C. elegans  along with sequence analysis and its functional expression and characterization. The phenotype changes were observed when  anp-1  mutated. Then, differential expression genes (DEGs) between wild type strain (N2) and  anp-1  deletion strain (RB804) were identified using transcriptome sequencing method. Finally, DEGs were verified by qRT-PCR assay.    Results  Our observations suggested that  anp-1  mutation induced small body size in the L4/young adult stage of  C. elegans , however, there was no difference between N2 and RB804 in adult stage. Moreover, deletion of  anp-1  resulted in shortening lifespan and laying fewer eggs. DEGs (184 genes) were observed between N2 groups and RB804 groups by transcriptome sequencing. According to GO annotations and KEGG enrichment analysis, these DEGs play vital roles in development regulation in  C. elegans . These data demonstrate ANP-1 participates in development and aging of  C. elegans  and will considerably contribute to the existing knowledge of aminopeptidase function in  C. elegans .

Characterization of aminopeptidase encoding gene anp-1 and its association with development in Caenorhabditis elegans