English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Myxococcus xanthus  displays a form of surface motility known as social (S) gliding. It is mediated by the type IV pilus (T4P) and requires the exopolysaccharide (EPS) to function. It is clear that T4P retraction powers S motility. EPS on a neighboring cell or deposited on a gliding surface is proposed to anchor the distal end of a pilus and trigger T4P retraction at its proximal end. Inversely, T4P has been shown to regulate EPS production upstream of the Dif signaling pathway. Here we describe the isolation of two Tn insertions at the  stk  locus which had been known to play roles in cellular cohesion and formation of cell groups. An insertion in  stkA  (MXAN_3474) was identified based on its ability to restore EPS to a  pilA  deletion mutant. The  stkA  encodes a DnaK or Hsp70 homolog and it is upstream of  stkB  (MXAN_3475) and  stkC  (MXAN_3476). A  stkB  insertion was identified in a separate genetic screen because it eliminated EPS production of an EPS +  parental strain. Our results with in-frame deletions of these three  stk  genes indicated that the  stkA  mutant produced increased level of EPS while  stkB  and  stkC  mutants produced less EPS relative to the wild type. S motility and developmental aggregation were affected by deletions of  stkA  and  stkB  but only minimally by the deletion of  stkC . Genetic epistasis indicated that StkA functions downstream of T4P but upstream of the Dif proteins as a negative regulator of EPS production in  M. xanthus .

The Hsp70-like StkA functions between T4P and Dif signaling proteins as a negative regulator of exopolysaccharide inMyxococcus xanthus